Anti-Human IFN Beta Antibody, Clone MMHB-3 (MAb)
- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
| Antigen | Human interferon beta |
| Isotype | IgG1 kappa |
| Host Species | Mouse |
| Purity | > 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg |
| Bioactivity | Measured by neutralization of interferon in cytopathic effect inhibition assay. |
| Specificity | Binds to human IFN-beta with high affinity |
| Storage | For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles |
| Antigen Synonyms | Human Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B |
| Clone | MMHB-3 |
Tech Info / Data
Tested Application:
- Direct Binding ELISA
Suggested Applications*:
- Western Blot
- Flow Cytometry
- Immunoprecipitation
- Immunohistochemistry
*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL
Citations
Documentation
21400-1 Certificate of Analysis (CoA) SDSRepresentative Binding Curve
Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 1% BSA/PBS (square) in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey and anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3",5,5"-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.
PBL的ELISA试剂盒是一整套范围广泛的异源抗体夹心免疫分析方法。该检测系统利用辣根过氧化物酶（HRP）和四甲基联苯胺（TMB）作为酶底物，可为干扰素和细胞因子的检测和定量提供灵敏而稳定的分析方法。
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